Common Genomic Response in Different Mouse Models of -Adrenergic–Induced Cardiomyopathy

Background—Although -adrenergic receptor (AR) blockade therapy is beneficial in the treatment of heart failure, little is known regarding the transcriptional mechanisms underlying this salutary action. Methods and Results—In the present study, we screened mice overexpressing Gs , 1AR, 2AR, or protein kinase A to test if a common genomic pathway exists in different models with enhanced -adrenergic signaling. In mice overexpressing Gs , differentially expressed genes were identified by mRNA profiling. In addition to well-known markers of cardiac hypertrophy (atrial natriuretic factor, CARP, and -myosin heavy chain), uncoupling protein 2 (UCP2), a protein involved in the control of mitochondrial membrane potential, and four-and-a-half LIM domain protein-1 (FHL1), a member of the LIM protein family, were predicted to be upregulated. Upregulation of these genes was confirmed by quantitative reverse transcriptase–polymerase chain reaction at all time points tested during the development of cardiomyopathy in mice overexpressing Gs . In mice overexpressing 1AR, 2AR, or protein kinase A, increased UCP2 and FHL1 expression was also observed at the onset of cardiomyopathy. AR blockade treatment reversed the cardiomyopathy and suppressed the increased expression of UCP2 and FHL1 in mice overexpressing Gs . Conclusions—UCP2 and FHL1 are important candidate genes that correlate with the development of AR-induced cardiomyopathy in different mouse models with enhanced AR signaling. In addition to preserving cardiac function, AR blockade treatment also prevents the genomic regulation that correlates with the onset of heart failure. (Circulation. 2003;108:2926-2933.)